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Figure S6. Treatment with a CCR4 antagonist decreases CCR4+ Tregs and increases T cell/Treg ratios in murine glioma. from CCL2 Produced by the Glioma Microenvironment Is Essential for the Recruitment of Regulatory T Cells and Myeloid-Derived Suppressor Cells

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posted on 2023-03-31, 00:31 authored by Alan L. Chang, Jason Miska, Derek A. Wainwright, Mahua Dey, Claudia V. Rivetta, Dou Yu, Deepak Kanojia, Katarzyna C. Pituch, Jian Qiao, Peter Pytel, Yu Han, Meijing Wu, Lingjiao Zhang, Craig M. Horbinski, Atique U. Ahmed, Maciej S. Lesniak
<p>(A-C) Vehicle control or C 021-treated mice were analyzed at 1 week post-GL261 injection for the accumulation of various T cell subsets and CD11b+Ly-6G+ MDSCs. (D-G) Quantification of flow cytometric analysis. (H-I) C 021 treatment improved the CD4/Foxp3 ratio and CD8/Foxp3 ratio. Data are representative of at least 2 independent experiments. Data are represented as mean {plus minus} SEM; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by Student's t test.</p>

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    DOI - Is supplement to CCL2 Produced by the Glioma Microenvironment Is Essential for the Recruitment of Regulatory T Cells and Myeloid-Derived Suppressor Cells

ARTICLE ABSTRACT

In many aggressive cancers, such as glioblastoma multiforme, progression is enabled by local immunosuppression driven by the accumulation of regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC). However, the mechanistic details of how Tregs and MDSCs are recruited in various tumors are not yet well understood. Here we report that macrophages and microglia within the glioma microenvironment produce CCL2, a chemokine that is critical for recruiting both CCR4+ Treg and CCR2+Ly-6C+ monocytic MDSCs in this disease setting. In murine gliomas, we established novel roles for tumor-derived CCL20 and osteoprotegerin in inducing CCL2 production from macrophages and microglia. Tumors grown in CCL2-deficient mice failed to maximally accrue Tregs and monocytic MDSCs. In mixed-bone marrow chimera assays, we found that CCR4-deficient Treg and CCR2-deficient monocytic MDSCs were defective in glioma accumulation. Furthermore, administration of a small-molecule antagonist of CCR4 improved median survival in the model. In clinical specimens of glioblastoma multiforme, elevated levels of CCL2 expression correlated with reduced overall survival of patients. Finally, we found that CD163-positive infiltrating macrophages were a major source of CCL2 in glioblastoma multiforme patients. Collectively, our findings show how glioma cells influence the tumor microenvironment to recruit potent effectors of immunosuppression that drive progression. Cancer Res; 76(19); 5671–82. ©2016 AACR.

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    Cancer Research

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    Cns CancersImmunologyCytokinesTumor resistance to immune response

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