posted on 2023-03-31, 00:11authored byJi Cao, Yijie Wang, Rong Dong, Guanyu Lin, Ning Zhang, Jing Wang, Nengming Lin, Yongchuan Gu, Ling Ding, Meidan Ying, Qiaojun He, Bo Yang
(A) Correlation between RhoGDI2 expression levels and metastatic potential in osteosarcoma patients. (B) Cells were exposed to hypoxia for 24 h or cells infected with control lentivirus (pCCL) and lentivirus-WSB1. RhoGDI2 mRNA expression was analyzed by RT-PCR and shown as a histogram after normalization, β-Actin was used as a control gene. (C) KHOS/NP cells treated with hypoxia for 24h together with 10 μM MG132, and RhoGDI2 protein levels were measured by western-blot analysis. (D) Colocalization of endogenous WSB1 and RhoGDI2. KHOS/NP cells were subjected to immunofluorescence staining with anti-WSB1 and anti-RhoGDI2 antibodies.