Figure S5. The small molecule inhibitor C 021 specifically inhibits Treg migration to CCR4 cognate chemokines. from CCL2 Produced by the Glioma Microenvironment Is Essential for the Recruitment of Regulatory T Cells and Myeloid-Derived Suppressor Cells

<p>(A) Sorted Tregs were placed the upper chamber of a 3.0 microm pore cell culture insert with or without 5 microM of the small molecule antagonist C 021 and chemokine-containing media was placed in the well. Migration index was determined after 4 hours through flow cytometric analysis. (B) A similar migration assay was performed as in A, except CCL21 and 10% FBS were used in the bottom chamber as a non-specific positive control. Data are representative of 3 independent experiments. Data are represented as mean {plus minus} SEM; *P < 0.05, by Student's t test.</p>