Figure S4 from A Subpopulation of Stromal Cells Controls Cancer Cell Homing to the Bone Marrow

<p>Supplementary-Figure 4 Absolute numbers of HSPCs and MSCs after G-CSF and G-CSF+PTH/ZA treatment and in Mx 1fl/fl knockout mice (A) The absolute number of HSPCs per femur was lower after treatment with G-CSF, whereas the number of MSCs was higher, n=12/14 in 3 experiments. (B) Addition of PTH/ZA to G-CSF did not affect HSPCs further, but reduced the absolute number of MSCs, n=14/14 in 3 experiments. (C) Conditional deletion of integrin 1 (Mx 1fl/fl) in the bone marrow had no effect on HSPCs, but led to increased absolute numbers of MSCs, n=6/9 in 2 experiments. Expression of MSC markers on low-passage human MSCs (D) In order to evaluate potential MSC markers, adherent human bone marrow cells (passages 0 or 1) were dissociated from the plates and stained for flow cytometry analysis. Shown is a graph summarizing the findings. In addition, in each panel the unstained control is shown on the left and the bone marrow staining on the right (E). The cells were 79% positive for CD31, 91% positive for CD44, 22% positive for CD56, 95% positive for CD73, 59% positive for CD140b, 61% positive for CD144, 72% positive for CD146, 87% positive for CD271, 87% positive for CD349, 90% positive for FAP, 90% positive for MSCA1, and 92% positive for Stro1; n=4 different aspirates.</p>