Figure S2 from Alkylating Agent–Induced NRF2 Blocks Endoplasmic Reticulum Stress–Mediated Apoptosis via Control of Glutathione Pools and Protein Thiol Homeostasis
posted on 2023-04-03, 15:47authored byAlfeu Zanotto-Filho, V. Pragathi Masamsetti, Eva Loranc, Sonal S. Tonapi, Aparna Gorthi, Xavier Bernard, Rosângela Mayer Gonçalves, José C.F. Moreira, Yidong Chen, Alexander J.R. Bishop
Effect of NAC, GSH-ethyl-ester or BSO on glutathione levels; Immunoblots for validation of NRF2-myc and GRP78-pcDNA3 overexpressions; mRNA levels of MRP and MDR gene expressions; Cell-titer Glo assays following inhibitors of MDR and MRP (verapamil and MK517) and thioredoxin reductase (auranofin); Immunoblots to demonstrate the purity of MDA-MB231 cell fractions; Dibromobimane assay showing that 10 mM DTT incubation does not restore Protein-SH levels depleted by MMS.