Figure 7 from Single-Cell Analysis of Bone Marrow CD8⁺ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine

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posted on 2024-12-04, 11:40 authored by Athanasios Tasis, Nikos E. Papaioannou, Maria Grigoriou, Nikolaos Paschalidis, Catherine Loukogiannaki, Anastasia Filia, Kyriaki Katsiki, Eleftheria Lamprianidou, Vasileios Papadopoulos, Christina Maria Rimpa, Antonios Chatzigeorgiou, Ioannis Kourtzelis, Petroula Gerasimou, Ioannis Kyprianou, Paul Costeas, Panagiotis Liakopoulos, Konstantinos Liapis, Petros Kolovos, Triantafyllos Chavakis, Themis Alissafi, Ioannis Kotsianidis, Ioannis Mitroulis

TF regulatory network analysis in BM-derived CD8⁺ T cells. A, UMAP depicting the clustering of CD8⁺ T cells based on regulons. B, Pie charts illustrating the representation of cells from CR and FAIL patients within each regulon. C, Comparison of cell distribution in regulons between the groups using separate UMAPs for each group. D, Heatmap showing the top differentially activated TFs of each regulon cluster. E and F, Violin plots depicting the activity score of selected TFs per sample type in regulons 5 and 7, respectively.

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ARTICLE ABSTRACT

Immunophenotypic analysis identified a BM CD57+CXCR3+ subset of CD8+ T cells associated with response to AZA in patients with MDS and AML. Single-cell RNA sequencing analysis revealed that IFN signaling is linked to the response to treatment, whereas TGF-β signaling is associated with treatment failure, providing insights into new therapeutic approaches.