Figure 7 from Determining the N-Glycan and Collagen/Extracellular Matrix Protein Compositions in a Novel Outcome Cohort of Prostate Cancer Tissue Microarrays Using MALDI-MSI

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posted on 2024-11-27, 09:40 authored by Jordan P. Hartig, Kaitlyn Bejar, Lyndsay E.A. Young, Grace Grimsley, Jennifer R. Bethard, Dean A. Troyer, Javier Hernandez, Jennifer D. Wu, Joseph E. Ippolito, Lauren E. Ball, Jonathan A.L. Gelfond, Teresa L. Johnson-Pais, Anand S. Mehta, Robin J. Leach, Peggi M. Angel, Richard R. Drake

Collagen/ECM MALDI-MSI data from full tissue samples (A) H&E-stained images of tissue from metastasis (MET) category and no recurrence (NED) category. Region where TMA was sampled is circled in black. Note that the MET tissue is the same H&E image used in Supplementary Fig. S8A, as N-glycans and collagenase peptides were detected sequentially on the same tissue slide. B, Corresponding MALDI-MSI images after digestion with COLaseIII from the two metastasis (MET) tissues (top) and the two no recurrence (NED) tissues (bottom). Corresponding m/z values for each image are shown to the right. C, Representative MALDI-MSI images from the metastasis (MET) TMA and no recurrence (NED) TMA of the same mass corresponding to full tissues. Note that the collagenase peptide image for m/z = 891.4323 is the same data image used in Fig. 5A for this ion.

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ARTICLE ABSTRACT

Using matrix-assisted laser desorption/ionization mass spectrometry imaging techniques on a unique cohort of prostate cancer tissues, we highlighted several molecular characteristics of matrix that have potential to act as early predictors of prostate cancer metastasis.