Figure 6 from Single-Cell Analysis of Bone Marrow CD8⁺ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine

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posted on 2024-12-04, 11:40 authored by Athanasios Tasis, Nikos E. Papaioannou, Maria Grigoriou, Nikolaos Paschalidis, Catherine Loukogiannaki, Anastasia Filia, Kyriaki Katsiki, Eleftheria Lamprianidou, Vasileios Papadopoulos, Christina Maria Rimpa, Antonios Chatzigeorgiou, Ioannis Kourtzelis, Petroula Gerasimou, Ioannis Kyprianou, Paul Costeas, Panagiotis Liakopoulos, Konstantinos Liapis, Petros Kolovos, Triantafyllos Chavakis, Themis Alissafi, Ioannis Kotsianidis, Ioannis Mitroulis

BM-derived CD8⁺ T cells of nonresponders (FAIL) displayed a suppressed cytotoxic molecular signature at the single-cell level. A, Dot plot representing MSigDB (Hallmark 2020) enrichment analysis of positively enriched pathways in FAIL patients. Enriched pathways with a q-value <0.05 (Benjamini–Hochberg correction) are shown. B, TGF-β signaling score of cluster 2 (CTL_1) and violin plots displaying the expression levels of the top DEGs of cluster 2 (CTL_1), involved in the enrichment of the TGF-β signaling pathway between CR and FAIL. C, TGF-β signaling score of cluster 4 (CTL_2) and violin plots displaying the expression levels of the top DEGs of cluster 4 (CTL_2), involved in the enrichment of the TGF-β signaling pathway between CR and FAIL. D, Comparison of the cytotoxic score of each group, as it is projected onto the respective UMAPs. E, Cytotoxic score of cluster 2 (CTL_1) and violin plots exhibiting the expression levels of the top differentially expressed cytotoxicity-related genes of cluster 2 (CTL_1) between CR and FAIL. F, Cytotoxic score of cluster 4 (CTL_2) and violin plots exhibiting the expression levels of the top differentially expressed cytotoxicity-related genes of cluster 4 (CTL_2) between CR and FAIL.

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ARTICLE ABSTRACT

Immunophenotypic analysis identified a BM CD57+CXCR3+ subset of CD8+ T cells associated with response to AZA in patients with MDS and AML. Single-cell RNA sequencing analysis revealed that IFN signaling is linked to the response to treatment, whereas TGF-β signaling is associated with treatment failure, providing insights into new therapeutic approaches.