Figure 5 from Single-Cell RNA Sequencing Unifies Developmental Programs of Esophageal and Gastric Intestinal Metaplasia

<p>Gastric and esophageal IM are enriched for extracellular matrix–depositing subtypes of fibroblasts. <b>A,</b> UMAP of fibroblast-like cells (cluster 13 in Supplementary Fig. S1) with fibroblast-specific clusters highlighted. <b>B,</b> UMAP of fibroblast-like cells (cluster 13 in Supplementary Fig. S1) with manually annotated cell types derived from Davidson et al. (<a href="#bib38" target="_blank">38</a>). <b>C,</b> UMAP of fibroblast-like cells (cluster 13 in Supplementary Fig. S1) with the contribution of individual tissue type highlighted. <b>D,</b> Bubble plot of top five genes identified in the differential analysis between the fibroblast-specific cell clusters. <b>E,</b> Bubble plot of genes identified as markers of early fibroblast development by Davidson et al. (<a href="#bib38" target="_blank">38</a>). <b>F,</b> Contribution of cell types derived from Davidson et al. (<a href="#bib38" target="_blank">38</a>) to cell counts from individual tissue types. The tissues originate from three studies: (i) Nowicki-Osuch and Zhuang et al. (<a href="#bib4" target="_blank">4</a>) and this study, (ii) Sathe et al. (<a href="#bib62" target="_blank">62</a>), and (iii) Zhang et al. (<a href="#bib61" target="_blank">61</a>). For tissue for which patient status could be identified, the tissue was split into healthy and disease states. NGB samples are adjacent to gastric cancer samples. <b>G,</b> Proportion of each subtype in total S1/S2/S3 fibroblasts derived from IF staining of E-GM, BE-IM, and GIM. <b>H,</b> Representative IF staining of E-GM, BE-IM, and GIM of S1 (CD34⁺CD31⁻), S2 (POSTN⁺aSMA⁻), and S3 (aSMA⁺) fibroblasts. <b>I,</b> Schematic summary of unified developmental trajectories between BE-IM and GIM.</p>