Figure 3 from Evaluating the Radiation Sensitivity Index and 12-Chemokine Gene Expression Signature for Clinical Use in a CLIA Laboratory

A, Impact of the amount of input RNA on GES scores. Three samples were profiled using different amounts of input RNA (100, 25, 10, 5, and 2.5 ng). Lower 12CK GES scores were observed at 2.5 ng, suggesting a lower limit on input RNA. RSI demonstrated more variability overall but did not seem to have a systematic difference at 2.5 ng. B, Impact of macrodissection on signature scores. Three samples were profiled across three different sample conditions: T, N and non-macrodissected tissue (PM). As expected, normal tissue can result in large changes to the signature score, which can be seen in PM as well. For instance, the 12CK GES score for normal tissue from sample 1 is much higher; however, there is elevated signal in the PM sample as well. By contrast, the differences in sample 3 (N, T, and PM) are small.