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Figure 2 from Evaluating the Radiation Sensitivity Index and 12-Chemokine Gene Expression Signature for Clinical Use in a CLIA Laboratory

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posted on 2025-03-03, 08:40 authored by Anders E. Berglund, John Puskas, Sean J. Yoder, Andrew T. Smith, Douglas C. Marchion, Dahui Qin, James J. Mulé, Javier F. Torres-Roca, Steven A. Eschrich

Replicability and precision analysis through measuring operator-to-operator and repeated run experiments. A, Operator variability: Three samples were run in duplicate by two different operators to assess both the variability in operator handling and repeatability from the same operator. The 12CK GES showed low variability across operator, whereas a larger difference in RSI score was obtained between operators. B, Repeated run over time: Four samples were repeated 1 week apart and assessed. The variability in the 12CK GES was low for all samples. RSI showed variability (less than 0.1) in two of the four samples.

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ARTICLE ABSTRACT

The RSI and 12CK GES are two GESs that predict tumor radiation sensitivity or the presence of tertiary lymphoid structures in tumors, respectively. These GESs were assessed within the CLIA process for future clinical use. We established proficiency, reproducibility, and reliability characteristics for both signatures in a controlled setting, indicating these GESs are suitable for validation within future clinical trials.