Figure 1 from Evaluating the Radiation Sensitivity Index and 12-Chemokine Gene Expression Signature for Clinical Use in a CLIA Laboratory

Proficiency and repeatability of the CLIA laboratory in generating 12CK GES and RSI. A, Proficiency of the CLIA laboratory compared with an established research-grade molecular genomics shared resource facility. Four samples were previously processed by the MCC Molecular Genomics Core (MGC) and available for CLIA laboratory processing. GESs were derived from both experimental conditions. The experiment was performed to determine that the CLIA laboratory was proficient in generating the expression data for the HG-U133Plus platform. Left, 12CK GES scores in MGC vs. CLIA laboratory (r = 0.991) indicating high correlation, although signature calibration was needed. Right, RSI signature scores in MGC vs. CLIA laboratory indicating compressed RSI signal from the CLIA experiments. B, Repeatability of GES from quadruplicate samples in the CLIA laboratory. Four samples were processed in quadruplicate and arrayed in the CLIA laboratory from the same operator. GES scores were derived from each experiment. Left, 12CK GES scores had a low variability in each of the four samples. Right, RSI was identical in two samples and had low level of variability in two samples.