Figure 1 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity

<p>Inflammatory cytokines induce heterogeneous TDO2 expression in TNBC cells. <b>A,</b> IHC of TDO2 protein in MDA-MB-453 cells treated with vehicle or 10 ng/mL IL1β + TNFα for 48 hours. <b>B,</b> TDO2 protein in MDA-MB-453 treated with IL1β + TNFα or vehicle control for 48 hours and detected by immunoblotting. <b>C,</b> t-SNE graph showing <i>TDO2</i> in scRNA-seq on MDA-MB-453 cells treated with vehicle or IL1β + TNFα for 48 hours. <b>D,</b> Volcano plot showing gene fold change following vehicle or IL1β + TNFα treatment (<i>P</i> < 0.05; FDR < 0.05) with twofold or greater up- (red) or downregulated (blue) gene sets. <b>E,</b> Significant upregulated/downregulated genes identified using GSEA. <b>F,</b> scRNA-seq expression of TRP catabolism–associated genes <i>TDO2</i>, <i>KMO</i>, and <i>KYNU</i> in MDA-MB-453 treated with vehicle or IL1β + TNFα. <b>G,</b><i>TDO2</i> measured by qRT-PCR in three TNBC cell lines in response to vehicle or IL1β + TNFα treatment. Data are displayed as mean ± SD by two-way ANOVA analysis. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001. <i>KMO</i>, <i>kynurenine 3</i>-<i>monooxygenase</i>; NES, normalized enrichment score; t-SNE, t-distributed stochastic neighbor embedding; UMAP, Uniform Manifold Approximation and Projection.</p>