American Association for Cancer Research
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Fig. S5 from Metastatic Conditioning of Myeloid Cells at a Subcutaneous Synthetic Niche Reflects Disease Progression and Predicts Therapeutic Outcomes

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posted on 2023-03-31, 03:22 authored by Robert S. Oakes, Grace G. Bushnell, Sophia M. Orbach, Pridvi Kandagatla, Yining Zhang, Aaron H. Morris, Matthew S. Hall, Petrina LaFaire, Joseph T. Decker, Rachel M. Hartfield, Michael D. Brooks, Max S. Wicha, Jacqueline S. Jeruss, Lonnie D. Shea

Experimental designs and OpenArray output for select genes with high fold-change and predictive value. (a) Results from OpenArray for TB mice at Days 7 (n=3), 14 (n=8), and 21 (n=3) along with time-matched TFC mice (n=14). (b) Results from OpenArray selected for fold change TFC or TB. For panels b-c mice are indicated on x-axis and genes are indicated on y-axis with clustering done via Euclidian distance and average linkage. Genes indicated in red text on the y-axis were selected for the smaller 10-gene panel analysis is subsequent studies.





Monitoring metastatic events in distal tissues is challenged by their sporadic occurrence in obscure and inaccessible locations within these vital organs. A synthetic biomaterial scaffold can function as a synthetic metastatic niche to reveal the nature of these distal sites. These implanted scaffolds promote tissue ingrowth, which upon cancer initiation is transformed into a metastatic niche that captures aggressive circulating tumor cells. We hypothesized that immune cell phenotypes at synthetic niches reflect the immunosuppressive conditioning within a host that contributes to metastatic cell recruitment and can identify disease progression and response to therapy. We analyzed the expression of 632 immune-centric genes in tissue biopsied from implants at weekly intervals following inoculation. Specific immune populations within implants were then analyzed by single-cell RNA-seq. Dynamic gene expression profiles in innate cells, such as myeloid-derived suppressor cells, macrophages, and dendritic cells, suggest the development of an immunosuppressive microenvironment. These dynamics in immune phenotypes at implants was analogous to that in the diseased lung and had distinct dynamics compared with blood leukocytes. Following a therapeutic excision of the primary tumor, longitudinal tracking of immune phenotypes at the implant in individual mice showed an initial response to therapy, which over time differentiated recurrence versus survival. Collectively, the microenvironment at the synthetic niche acts as a sentinel by reflecting both progression and regression of disease. Immune dynamics at biomaterial implants, functioning as a synthetic metastatic niche, provides unique information that correlates with disease progression.