American Association for Cancer Research
crc-22-0494_fig7.png (370.72 kB)

FIGURE 7 from Surgical Inflammation Alters Immune Response to Intraoperative Photodynamic Therapy

Download (370.72 kB)
posted on 2023-09-11, 14:20 authored by Richard W. Davis, Astero Klampatsa, Gwendolyn M. Cramer, Michele M. Kim, Joann M. Miller, Min Yuan, Cassandra Houser, Emma Snyder, Mary Putt, Sergei A. Vinogradov, Steven M. Albelda, Keith A. Cengel, Theresa M. Busch

T cells and splenocyte cells from TI/PDT treated mice limit PDT-induced antitumor immunity in a C57BL/6 model of mesothelioma. Murine mesothelioma AE17o cells were implanted into the flank of C57BL/6 mice and grown to 80 mm3 prior to treating with TI and/or PDT. A, Tumor responses to PDT (n = 14) were impeded by exposure to TI prior to PDT (n = 15; P = 0.019 for PDT vs. TI/PDT). Transferability of antitumor immunity in AE17o tumors was assessed using CD8+ T cells isolated from splenocytes (B), or using whole splenocyte populations, from tumored, PDT-, or TI/PDT-treated mice (C). T cells or splenocytes were transferred alongside AE17o tumor cells to recipient mice. Preceding PDT with TI reduced the antitumor immunity encoded in CD8+ T cells (P = 0.0364 for TI/PDT vs. PDT) and whole splenocytes (P = 0.0017 for TI/PDT vs. PDT). Growth delay analysis for AE17o tumors represents growth to 50 mm3 for T cell and 175 mm3 for splenocyte transfer. n = 5 recipient mice/group; *, P < 0.05; **, P < 0.01; ***, P < 0.001.


HHS | NIH | National Cancer Institute (NCI)



Although mesothelioma is difficult to treat, we have shown that combining surgery with a form of radiation, photodynamic therapy, may help people with mesothelioma live longer. In this study, we demonstrate in mice that this regimen could be further improved by addressing the inflammation induced as a by-product of surgery.