FIGURE 7 from Surgical Inflammation Alters Immune Response to Intraoperative Photodynamic Therapy
T cells and splenocyte cells from TI/PDT treated mice limit PDT-induced antitumor immunity in a C57BL/6 model of mesothelioma. Murine mesothelioma AE17o cells were implanted into the flank of C57BL/6 mice and grown to 80 mm3 prior to treating with TI and/or PDT. A, Tumor responses to PDT (n = 14) were impeded by exposure to TI prior to PDT (n = 15; P = 0.019 for PDT vs. TI/PDT). Transferability of antitumor immunity in AE17o tumors was assessed using CD8+ T cells isolated from splenocytes (B), or using whole splenocyte populations, from tumored, PDT-, or TI/PDT-treated mice (C). T cells or splenocytes were transferred alongside AE17o tumor cells to recipient mice. Preceding PDT with TI reduced the antitumor immunity encoded in CD8+ T cells (P = 0.0364 for TI/PDT vs. PDT) and whole splenocytes (P = 0.0017 for TI/PDT vs. PDT). Growth delay analysis for AE17o tumors represents growth to 50 mm3 for T cell and 175 mm3 for splenocyte transfer. n = 5 recipient mice/group; *, P < 0.05; **, P < 0.01; ***, P < 0.001.