FIGURE 7 from Integrative Metatranscriptomic Analysis Reveals Disease-specific Microbiome–host Interactions in Oral Squamous Cell Carcinoma

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posted on 2023-05-08, 14:20 authored by Vinay Jain, Divyashri Baraniya, Doaa E. El-Hadedy, Tsute Chen, Michael Slifker, Fadhl Alakwaa, Kathy Q. Cai, Kumaraswamy N. Chitrala, Christopher Fundakowski, Nezar N. Al-Hebshi

Preliminary in vitro experimental validation in SCC25 cells. A,Cutibacterium acnes and the MYC gene were chosen for this experiment based on their strong correlation in the sequencing data. r_s, Spearman correlation coefficient; CLR, centered log ratio; Rlog, regularized log transformation defined in the DESeq2 package. B,C. ances NCTC 737 grown to mid-log phase was cocultured with SCC25 cells at MOI of 50, 100, and 200 for 24 hours. Levels of MYC mRNA were measured by qRT-PCR, normalized to GADPH and relative to the noninfected cells (controls). * Statistically significant, Welch-corrected t test.

Funding

HHS | NIH | National Institute of Dental and Craniofacial Research (NIDCR)

Dr. Cary. R. Klimen Oral Health Sciences Research Program Fund

History

ARTICLE ABSTRACT

Studies have shown that a distinct microbiome is associated with OSCC, but how the microbiome functions within the tumor interacts with the host cells remains unclear. By simultaneously characterizing the microbial and host transcriptomes in OSCC and control tissues, the study provides novel insights into microbiome-host interactions in OSCC which can be validated in future mechanistic studies.