American Association for Cancer Research
crc-23-0102_fig6.png (1.4 MB)

FIGURE 6 from Sequential Chromogenic IHC: Spatial Analysis of Lymph Nodes Identifies Contact Interactions between Plasmacytoid Dendritic Cells and Plasmablasts

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posted on 2023-07-21, 17:40 authored by Natalie Claudio, My-Tien Nguyen, Adrian Wanner, Ferdinando Pucci

Citrate stripping is necessary before EDTA-based antigen retrieval. A patient sample was stained with CD68 in cycle 11 round 2 (A). In the next cycle, an EDTA antigen retrieval buffer was used to detect BCL6, without citrate stripping. Carryover from the previous cycle was observed by treatment with AEC and visualization (B). Stripping with a citrate antigen retrieval buffer was performed and EDTA-based antigen retrieval was conducted again, which allowed to visualize BCL6 without carryover from previous staining (C). Note the different morphology of BCL6+ and CD68+ cells within the same region (A, C). Same section is shown in A–C. Scale bar: 100 μm (10X).


V Foundation for Cancer Research (VFCR)

Cancer Research UK (CRUK)



We present a comprehensive guide for developing customized biomarker panels to investigate cell–cell interactions in the context of immune responses in cancer. This approach revealed novel contact interactions between plasmablasts and plasmacytoid dendritic cells in lymph nodes from patients with head and neck cancer.