FIGURE 3 from Nanoparticle Delivery of Immunostimulatory Alu RNA for Cancer Immunotherapy

In vitro characterization of AluJb RNA (Left Arm)/D-PDB. A, Agarose gel of AluJb RNA (Left Arm) with and without various amounts of D-PDB, as indicated. 1 µg RNA/lane. The TrackIt 100 bp DNA Ladder was employed. B, DLS analysis of Alu-NPs and Edited Alu-NPs (i.e., AluJb RNA (Left Arm)/D-PDB at an N/P ratio of 4 and Inactive AluJB (Left Arm)/D-PDB at an N/P ratio of 4) relative to D-PDB. C, TEM images of D-PDB NPs, Edited Alu-NPs, and Alu-NPs. Scale bars, 100 µm. RAW-Dual (D) and THP1-Dual (E) reporter cell assays of Alu-NPs, Edited Alu-NPs, D-PDB, D-B Alu NPs, and Free AluJB RNA (Left Arm). All curves are normalized to the maximum value of the Alu-NPs. All experiments were performed at least twice.