American Association for Cancer Research
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FIGURE 3 from Effects and Eradication of Mycoplasma Contamination on Patient-derived Colorectal Cancer Organoid Cultures

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posted on 2023-09-27, 19:20 authored by Divya L. Dayanidhi, Wylie K. Watlington, John B. Mantyh, Gabrielle Rupprecht, David S. Hsu

Mycoplasma contamination can change organoid drug sensitivity. A, Dose–response curves for four sets of Mycoplasm-positive and -negative lines for three drugs: oxaliplatin, SN38, and 5-FU. *, P < 0.05 (Mann–Whitney). B, High-throughput screens for three sets of Mycoplasm-positive and -negative organoid lines. Percent killing for each drug is indicated by color, with red being the highest percent killing and blue being the lowest. C, Difference in percent killing in high-throughput screen between Mycoplasm-negative and -positive organoid lines.


HHS | National Institutes of Health (NIH)



Organoids are valuable models of cancer. Mycoplasma contamination can alter organoid drug sensitivity, so there is a need for a standardized protocol to detect and remove Mycoplasma from organoids. We report a simple procedure for removing Mycoplasma from organoids via in vivo passaging through mice followed by re-establishment of organoids.