FIGURE 2 from Tumor-infiltrating Leukocyte Profiling Defines Three Immune Subtypes of NSCLC with Distinct Signaling Pathways and Genetic Alterations

Immune subtypes based on TIL profiling using FCM; NAT (n = 157), LUAD [85; Cold (n = 19), Myeloid (n = 36), CD8 (n = 30)], LUSQ [50; Cold (n = 14), Myeloid (n = 19), CD8 (n = 17)]. A, Heat map of cell densities and �45 of immune cells in NSCLC tumors and NATs. Each column represents a patient; data were normalized by Z-scores to indicate relative compositions of immune cells. Cell density is presented as the log of each cell type. �45 is presented as each immune cell type/CD45. Heat maps of cell density and �45 of TILs in LUAD (B) and LUSQ (C) tissues. D, Cell densities and �45 of immune cell types in LUAD and LUSQ immune subtypes. Top, Cell density indicates immune cells. Bottom, �45 indicates immune cells. AD.Cold, AD.Myeloid, and AD.CD8 are LUAD subtypes Cold, Myeloid, and CD8, respectively. SQ.Cold, SQ.Myeloid, and SQ.CD8 are LUSQ subtypes Cold, Myeloid, and CD8, respectively. E, IHC staining for CD8 in a representative case from immune subtypes. For CD8, FOXP3, CD20, and Ki67, the positivity of each marker was given as the number of total positive cell number within each tissue core. For CD33, the ratio of CD33-positive staining area per tissue area was calculated. F, Immune cell counts by IHC staining in lung cancer tissues. G, CD8⁺ T cell–attractant chemokine gene expression in immune subtypes.