American Association for Cancer Research
crc-23-0109_fig2.png (796.3 kB)

FIGURE 2 from Effects and Eradication of Mycoplasma Contamination on Patient-derived Colorectal Cancer Organoid Cultures

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posted on 2023-09-27, 19:20 authored by Divya L. Dayanidhi, Wylie K. Watlington, John B. Mantyh, Gabrielle Rupprecht, David S. Hsu

Mycoplasma can be successfully cleared from PDO lines by in vivo passaging through immunodeficient mice. A,Mycoplasma test for organoid lines before (+) and after (−) in vivo passaging. Representative positive and negative control PCR products are included within lanes 1 and 2 of the gel on the left. The top band may not appear if the original sample contained a high amount of Mycoplasma. B, Growth comparison of Mycoplasma-positive and -negative lines over 12 days. Images on top compare PDO growth between positive and negative lines on days 0 and 12. Graphs on the bottom show CTG fluorescence of the PDO taken every other day for 12 days.


HHS | National Institutes of Health (NIH)



Organoids are valuable models of cancer. Mycoplasma contamination can alter organoid drug sensitivity, so there is a need for a standardized protocol to detect and remove Mycoplasma from organoids. We report a simple procedure for removing Mycoplasma from organoids via in vivo passaging through mice followed by re-establishment of organoids.