FIGURE 1 from Targeting Fatty Acid Reprogramming Suppresses CARM1-expressing Ovarian Cancer

CARM1 regulates genes involved in FA metabolism. A, Expression of CARM1 and a loading control β-actin in control and CARM1 knockout (KO) A1847 cells determined by immunoblot. B, Scatter plot of KEGG pathway analysis for genes upregulated in wildtype compared with CARM1 KO A1847 cells (WT/KO >2, FDR < 0.05). Dot size represents gene number, color represents P value. P values were calculated by KEGG analysis. C, Volcano plot illustrating the protein expression detected by RPPA in wildtype or CARM1 KO A1847 cell lysates. Red dots and green dots indicate protein expression significantly upregulated in wildtype and knockout cells, respectively. SCD1, FASN, and ACC1 were highlighted in blue. P value was calculated using a two-tailed Student t test. D, Schematic of the MUFA synthesis pathway. E, mRNA expression of ACC1, FASN, and SCD1 in control and CARM1 KO A1847 cells determined by qRT-PCR analysis. P value was calculated using a two-tailed Student t test. F, Expression of SCD1, FASN, ACC1 and a loading control β-actin in control and CARM1 KO A1847 cells as determined by immunoblot. G, Expression of SCD1, FASN, ACC1 and a loading control β-actin in control and CARM1 overexpressed CAOV3 cells as determined by immunoblot. P value was calculated using a two-tailed Student t test. Data represent mean ± SEM, n = 3 biologically independent experiments.