FIGURE 1 from An Early Neoplasia Index (ENI10), Based on Molecular Identity of CD10 Cells and Associated Stemness Biomarkers, is a Predictor of Patient Outcome in Many Cancers

BMP2 induces early transformation of a model of breast SCs and increases CD10 expression. A, Schematic representation of the experimental protocol used to obtain the MCF10-CT, MC26, and M1B26 cell lines from the MCF10A cells. B, Quantification of soft-agar colony formation [error bars represent the SD (n = 7), significance measured using the Mann–Whitney test is indicated on the graph by the P values. ns for P > 0.05]. C, Xenografts of the indicated number cells of MCF10A-derived models injected into nude mice presented as the number of successful grafts after 4 weeks/mouse (n = 10). D, GSEA of transcriptomic data comparing MC26 cells (top row) or M1B26 cells (bottom row) with MCF10A-CT cells. Data represent enrichment plots analyzed using public gene sets (41) of upregulated (left) or downregulated (right) genes in human primary ductal carcinoma compared with healthy tissues. E, GSEA of transcriptomic data comparing MC26 (first column) or M1B26 (second column) with CT cells and M1B26 with MC26 cells (third column). The “hallmarks” gene sets from the MSigDB were used and the NES (normalized enrichment score) with P values inferior to 0.05 are shown. F, E-CFC Progenitor content from MCF10A-CT cells (n = 5), MC26 cells (n = 8) or M1B26 cells (n = 6) quantified after 6 days by scoring colonies numbers and presented/10,000 cells. G, Number of spheres per 100 seeded cells after 1 week from MCF10A-CT cells (n = 4), MC26 cells (n = 6), and M1B26 cells (n = 6). H, TDLU, 3D structures from primary human breast cells (top) and MCF10A cell line (bottom). I, Images at day 21 of 3D structures in the TDLU assay from MCF10A-CT, MC26, or M1B26 cells. A representative TDLU section from MCF10A-CT, stained with H&E, is shown on the top right. J, Flow cytometry analysis of CD10 expression on MCF10A-CT (n = 4), MC26 (n = 7), and M1B26 (n = 6) presented as the percentage of positive cells (left) and mean fluorescence intensity (right).