American Association for Cancer Research
21598290cd190329-sup-219026_2_supp_5782671_py1666.xlsx (9.99 kB)

Table S4 from The Phenotypes of Proliferating Glioblastoma Cells Reside on a Single Axis of Variation

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posted on 2023-04-03, 22:43 authored by Lin Wang, Husam Babikir, Sören Müller, Garima Yagnik, Karin Shamardani, Francisca Catalan, Gary Kohanbash, Beatriz Alvarado, Elizabeth Di Lullo, Arnold Kriegstein, Sumedh Shah, Harsh Wadhwa, Susan M. Chang, Joanna J. Phillips, Manish K. Aghi, Aaron A. Diaz

Table S4: Antibodies used for IHC.


UCSF Glioma Precision Medicine Program

UC Cancer Research Coordinating Committee



Although tumor-propagating cells can be derived from glioblastomas (GBM) of the proneural and mesenchymal subtypes, a glioma stem-like cell (GSC) of the classic subtype has not been identified. It is unclear whether mesenchymal GSCs (mGSC) and/or proneural GSCs (pGSC) alone are sufficient to generate the heterogeneity observed in GBM. We performed single-cell/single-nucleus RNA sequencing of 28 gliomas, and single-cell ATAC sequencing for 8 cases. We found that GBM GSCs reside on a single axis of variation, ranging from proneural to mesenchymal. In silico lineage tracing using both transcriptomics and genetics supports mGSCs as the progenitors of pGSCs. Dual inhibition of pGSC-enriched and mGSC-enriched growth and survival pathways provides a more complete treatment than combinations targeting one GSC phenotype alone. This study sheds light on a long-standing debate regarding lineage relationships among GSCs and presents a paradigm by which personalized combination therapies can be derived from single-cell RNA signatures, to overcome intratumor heterogeneity. Tumor-propagating cells can be derived from mesenchymal and proneural glioblastomas. However, a stem cell of the classic subtype has yet to be demonstrated. We show that classic-subtype gliomas are comprised of proneural and mesenchymal cells. This study sheds light on a long-standing debate regarding lineage relationships between glioma cell types.See related commentary by Fine, p. 1650.This article is highlighted in the In This Issue feature, p. 1631

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