dataset posted on 2023-03-31, 01:00 authored by Wei Du, Xing Xu, Qing Niu, Xuexi Zhang, Yiliang Wei, Ziqiao Wang, Wei Zhang, Jun Yan, Yongxin Ru, Zheng Fu, Xiaobo Li, Yuan Jiang, Zhenyi Ma, Zhenfa Zhang, Zhi Yao, Zhe Liu
Table S2, related to Figure 4: Genes downregulated/upregulated >2-fold by Spi-B transient expression in A549 cells
National Natural Science Foundation of China
Ministry of Science and Technology of China
China Postdoctoral Science Foundation
ARTICLE ABSTRACTDissociation from epithelial sheets and invasion through the surrounding stroma are critical early events during epithelial cancer metastasis. Here we find that a lymphocyte lineage–restricted transcription factor, Spi-B, is frequently expressed in human lung cancer tissues. The Spi-B–expressing cancer cells coexpressed vimentin but repressed E-cadherin and exhibited invasive behavior. Increased Spi-B expression was associated with tumor grade, lymphatic metastasis, and short overall survival. Mechanistically, Spi-B disrupted intercellular junctions and enhanced invasiveness by reconfiguring the chromatin structure of the tight junction gene claudin-2 (CLDN2) and repressing its transcription. These data suggest that Spi-B participates in mesenchymal invasion, linking epithelial cancer metastasis with a lymphatic transcriptional program. Cancer Res; 77(18); 4809–22. ©2017 AACR.