dataset
posted on 2023-12-01, 08:23 authored by Donald T. Weed, Serena Zilio, Christie McGee, Boutheina Marnissi, Zoukaa Sargi, Elizabeth Franzmann, Giovana Thomas, Jason Leibowitz, Elizabeth Nicolli, David Arnold, Silvio Bicciato, Paolo Serafini <p>Numeric data describing a) sample composition, analysis parameters, and phenotyping; b)
phenotype composition of the merged dataset and of each single sample; c) cell type composition (number of
cells) of cellular neighborhoods identified at W=10 and K=14; d) Number of cells assigned to each of the 11
cellular neighborhoods identified at W=10 and K=14; e): relative cell-cell contact frequencies (RF) for all
phenotypes in TLS1 and TLS2 CNs across all patients; f) relative cell-cell contact frequencies (RF) for all
phenotypes in hot and cold tumor CNs across all patients</p>
Funding
Fondazione AIRC per la ricerca sul cancro ETS (AIRC)
Sylvester Comprehensive Cancer Center, University of Miami Health Systems (Sylvester Comprehensive Cancer Center)
Cancer Moonshot (Misión contra el Cáncer)
History
ARTICLE ABSTRACT
Emerging evidence suggests that not only the frequency and composition of tumor-infiltrating leukocytes but also their spatial organization might be a major determinant of tumor progression and response to therapy. Therefore, mapping and analyzing the fine tumor immune architecture could potentially provide insights for predicting cancer prognosis. Here, we performed an explorative, prospective clinical study to assess whether structures within the tumor microenvironment can predict recurrence after salvage surgery in head and neck squamous cell carcinoma (HNSCC). The major immune subsets were measured using flow cytometry and co-detection by indexing (CODEX) multiparametric imaging. Flow cytometry underestimated the number of PMN-MDSCs and neutrophils in the tumor and overestimated the tumor-infiltrating lymphocyte frequency. An ad hoc computational framework was used to identify and analyze discrete cellular neighborhoods. A high frequency of tertiary lymphoid structures composed of CD31highCD38high plasma cells was associated with reduced recurrence after surgery in HNSCC. These data support the notion that the structural architecture of the tumor immune microenvironment plays an essential role in tumor progression and indicates that type 1 tertiary lymphoid structures and long-lived CD31highCD38high plasma cells are associated with good prognosis in HNSCC.
Imaging the spatial tumor immune microenvironment and evaluating the presence of type 1 tertiary lymphoid structures enables prediction of recurrence after surgery in patients with head and neck squamous cell carcinoma.
