American Association for Cancer Research
ccr-23-0219_supplementary_table_s7_suppts7.xlsx (10.36 kB)

Supplementary Table S7 from Activin A–Mediated Polarization of Cancer-Associated Fibroblasts and Macrophages Confers Resistance to Checkpoint Immunotherapy in Skin Cancer

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posted on 2023-09-01, 08:20 authored by Christine Pich-Bavastro, Laura Yerly, Jeremy Di Domizio, Stéphanie Tissot-Renaud, Michel Gilliet, François Kuonen

Supplementary Table S7. Clinical response annotation of the scRNAseq dataset obtained from melanomas treated with ICI.


Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF)

Faculté de Biologie et de Médecine, Université de Lausanne (FBM)

Promedica Stiftung



Cemiplimab is approved for the treatment of locally advanced basal cell carcinomas (BCC), although with mitigated results. We sought to interrogate the cellular and molecular transcriptional reprogramming underlying BCC resistance to immunotherapy. Here, we combined spatial and single-cell transcriptomics to deconvolute the spatial heterogeneity of the tumor microenvironment in regard with response to immunotherapy, in a cohort of both naïve and resistant BCCs. We identified subsets of intermingled cancer-associated fibroblasts (CAF) and macrophages contributing the most to CD8 T-cell exclusion and immunosuppression. Within this spatially resolved peritumoral immunosuppressive niche, CAFs and adjacent macrophages were found to display Activin A–mediated transcriptional reprogramming towards extracellular matrix remodeling, suggesting active participation to CD8 T-cell exclusion. In independent datasets of human skin cancers, Activin A–conditioned CAFs and macrophages were associated with resistance to immune checkpoint inhibitors (ICI). Altogether, our data identify the cellular and molecular plasticity of tumor microenvironment (TME) and the pivotal role of Activin A in polarizing the TME towards immune suppression and ICI resistance.

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