American Association for Cancer Research
00085472can152120-sup-153620_3_supp_3376129_2342r2.xlsx (30.57 kB)

Supplementary Table S5 from miR-146b-5p within BCR-ABL1–Positive Microvesicles Promotes Leukemic Transformation of Hematopoietic Cells

Download (30.57 kB)
posted on 2023-03-30, 23:43 authored by Hong-Mei Zhang, Qing Li, Xiaojian Zhu, Wei Liu, Hui Hu, Teng Liu, Fanjun Cheng, Yong You, Zhaodong Zhong, Ping Zou, Qiubai Li, Zhichao Chen, An-Yuan Guo

Supplementary Table S5. GO enrichment for 8 cluters of DEGs


National Natural Science Foundation of China (NSFC)

National Natural Science Foundation of China

New Century Excellent Talents in University (NCET)



Evidence is accumulating that extracellular microvesicles (MV) facilitate progression and relapse in cancer. Using a model in which MVs derived from K562 chronic myelogenous leukemia (CML) cells transform normal hematopoietic transplants into leukemia-like cells, we defined the underlying mechanisms of this process through gene-expression studies and network analyses of transcription factors (TF) and miRNAs. We found that antitumor miRNAs were increased and several defense pathways were initiated during the early phases of oncogenic transformation. Later, oncomiRs and genes involved in cell cycle, DNA repair, and energy metabolism pathways were upregulated. Regulatory network analyses revealed that a number of TFs and miRNAs were responsible for the pathway dysregulation and the oncogenic transformation. In particular, we found that miR-146b-5p, which was highly expressed in MVs, coordinated the regulation of cancer-related genes to promote cell-transforming processes. Notably, treatment of recipient cells with MV derived from K562 cells expressing mimics of miR-146b-5p revealed that it accelerated the transformation process in large part by silencing the tumor-suppressor NUMB. High levels of miR-146b-5p also enhanced reactive oxygen species levels and genome instability of recipient cells. Taken together, our finding showed how upregulation of oncogenic miRNAs in MVs promote hematopoetic cells to a leukemic state, as well as a demonstration for TF and miRNA coregulatory analysis in exploring the dysregulation of cancers and discovering key factors. Cancer Res; 76(10); 2901–11. ©2016 AACR.