American Association for Cancer Research

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Supplementary Table S5 from Chemotherapy Induces Tumor-Associated Macrophages that Aid Adaptive Immune Responses in Ovarian Cancer

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posted on 2023-04-04, 01:01 authored by Owen Heath, Chiara Berlato, Eleni Maniati, Anissa Lakhani, Colin Pegrum, Panoraia Kotantaki, Samar Elorbany, Steffen Böhm, Simon T. Barry, Alessandro Annibaldi, Desmond P. Barton, Frances R. Balkwill

List of GSEA canonical pathways and gene ontologies in AZD vs Ctrl


Barts Gynae Tissue Bank

BCI Flow Cytometry Facility

Cancer Research UK

Wellcome Trust

Well Being of Women

Barts Charity



Neoadjuvant chemotherapy (NACT) may stimulate anticancer adaptive immune responses in high-grade serous ovarian cancer (HGSOC), but little is known about effects on innate immunity. Using omental biopsies from HGSOC, and omental tumors from orthotopic mouse HGSOC models that replicate the human tumor microenvironment, we studied the impact of platinum-based NACT on tumor-associated macrophages (TAM). We found that chemotherapy reduces markers associated with alternative macrophage activation while increasing expression of proinflammatory pathways, with evidence of inflammasome activation. Further evidence of a shift in TAM functions came from macrophage depletion via CSF1R inhibitors (CSF1Ri) in the mouse models. Although macrophage depletion in established disease had no impact on tumor weight or survival, CSF1Ri treatment after chemotherapy significantly decreased disease-free and overall survival. This decrease in survival was accompanied by significant inhibition of adaptive immune response pathways in the tumors. We conclude that chemotherapy skews the TAM population in HSGOC toward an antitumor phenotype that may aid adaptive immune responses, and therapies that enhance or sustain this during remission may delay relapse.