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Supplementary Table 26 from Unraveling the Global Proteome and Phosphoproteome of Prostate Cancer Patient-Derived Xenografts

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posted on 2024-05-02, 08:06 authored by Zoi E. Sychev, Abderrahman Day, Hannah E. Bergom, Gabrianne Larson, Atef Ali, Megan Ludwig, Ella Boytim, Ilsa Coleman, Eva Corey, Stephen R. Plymate, Peter S. Nelson, Justin H. Hwang, Justin M. Drake

Supplementary Table 26. LuCaP Proteome VSN Normalized Differential Expression ROTS AdCa CR vs AdCa NCR

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U.S. Department of Defense (DOD)

National Institutes of Health (NIH)

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ARTICLE ABSTRACT

Resistance to androgen-deprivation therapies leads to metastatic castration-resistant prostate cancer (mCRPC) of adenocarcinoma (AdCa) origin that can transform into emergent aggressive variant prostate cancer (AVPC), which has neuroendocrine (NE)-like features. In this work, we used LuCaP patient-derived xenograft (PDX) tumors, clinically relevant models that reflect and retain key features of the tumor from advanced prostate cancer patients. Here we performed proteome and phosphoproteome characterization of 48 LuCaP PDX tumors and identified over 94,000 peptides and 9,700 phosphopeptides corresponding to 7,738 proteins. We compared 15 NE versus 33 AdCa samples, which included six different PDX tumors for each group in biological replicates, and identified 309 unique proteins and 476 unique phosphopeptides that were significantly altered and corresponded to proteins that are known to distinguish these two phenotypes. Assessment of concordance from PDX tumor-matched protein and mRNA revealed increased dissonance in transcriptionally regulated proteins in NE and metabolite interconversion enzymes in AdCa. Overall, our study highlights the importance of protein-based identification when compared with RNA and provides a rich resource of new and feasible targets for clinical assay development and in understanding the underlying biology of these tumors.

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